Journal: FEMS Microbiology Letters

Article Title: Characterization of the L-form switch in the Gram-negative pathogen Streptobacillus moniliformis

doi: 10.1093/femsle/fnab156

Figure Lengend Snippet: Growth and morphology of S. moniliformis . (A) Growth of S. moniliformis type strain 9901 on NA plate containing 20% calf serum at 30°C. (B ) Phase-contrast micrographs of S. moniliformis in NB with serum after 1 or 4 days of incubation at 30°C. (C) Streptobacillus moniliformis cells were cultured in NB with serum in the CellASIC ONIX microfluidic system for time-lapse microscopy. Individual frames are extracted from Video S1 (Supporting Information). Numbers in the top right corner of each frame represent time (min) elapsed in the video. Arrowheads represent spontaneous formation of L-form-like structures.

Article Snippet: During the time lapse, media perfusion was maintained at 2 psi and the temperature at 32°C, with images acquired every 10 min. All flow rate and media switching steps were controlled using the CellASIC ONIX FG software (v 5.5.1.0).

Techniques: Incubation, Cell Culture, Time-lapse Microscopy

Journal: FEMS Microbiology Letters

Article Title: Characterization of the L-form switch in the Gram-negative pathogen Streptobacillus moniliformis

doi: 10.1093/femsle/fnab156

Figure Lengend Snippet: Effects of hypertonic solutions on growth and morphology of S. moniliformis . (A) Growth of S. moniliformis on NA plate containing serum supplemented with or without 0.5 M sucrose (MSM), glucose, succinate, NaCl or sorbitol at 30°C. (B) Phase-contrast micrograph of S. moniliformis in NB containing serum and MSM (0.5 M sucrose) at 30°C. (C) Streptobacillus moniliformis cells were cultured in NB containing serum and MSM in the CellASIC ONIX microfluidic system for time-lapse microscopy. Individual frames are extracted from Video S2 (Supporting Information). Numbers in the top right corner of each frame represent time (min) elapsed in the video.

Article Snippet: During the time lapse, media perfusion was maintained at 2 psi and the temperature at 32°C, with images acquired every 10 min. All flow rate and media switching steps were controlled using the CellASIC ONIX FG software (v 5.5.1.0).

Techniques: Cell Culture, Time-lapse Microscopy

Journal: FEMS Microbiology Letters

Article Title: Characterization of the L-form switch in the Gram-negative pathogen Streptobacillus moniliformis

doi: 10.1093/femsle/fnab156

Figure Lengend Snippet: Membrane growth of S. moniliformis L-forms in a liquid medium. (A) Streptobacillus moniliformis cells in NB with serum were treated with 200 μg/ml ampicillin in the CellASIC ONIX microfluidic system for time-lapse microscopy. Individual frames are extracted from Video S4 (Supporting Information). Numbers in the top left corner of each frame represent time (min) elapsed in the video. Arrowheads represent spontaneous formation of L-form-like structures. Arrow represents OM expansion followed by growth and deformations of L-form cell. (B) Phase-contrast micrographs of S. moniliformis L-forms during time-lapse experiments. Individual frames are extracted from Video S5 (Supporting Information). Numbers in the top left corner of each frame represent time (min) elapsed in the video. Red arrows show expansion of OM. Blue arrows show growth and deformation of cytoplasmic compartment. Yellow arrowheads show scission events.

Article Snippet: During the time lapse, media perfusion was maintained at 2 psi and the temperature at 32°C, with images acquired every 10 min. All flow rate and media switching steps were controlled using the CellASIC ONIX FG software (v 5.5.1.0).

Techniques: Time-lapse Microscopy